The formation of single-stranded breaks in DNA following UV irradiation is assessed in uvrC34 mutants. By altering the SOS DNA-repair system, either by additional mutations or by using drugs affecting transcription or translation, it is shown that such single-stranded breaks require one or more DNA-damage-inducible functions. A UV-sensitive strain is characterized as carrying a Tn10 insertion into the uvrC gene. The absence of post-irradiation incision in this strain demonstrates that uvrC function is absolutely required in vivo for the incision stage of excision repair, and suggests that other uvrC mutants are 'leaky'.

Original publication

DOI

10.1099/00221287-134-2-403

Type

Journal article

Journal

J Gen Microbiol

Publication Date

02/1988

Volume

134

Pages

403 - 412

Keywords

Bacterial Proteins, DNA Repair, DNA Transposable Elements, DNA, Bacterial, Escherichia coli, Genes, Bacterial, Mutation, SOS Response (Genetics), Ultraviolet Rays